{"text_format":"3","workbook":"xls","n_sheets":13,"sheets":["Table 1","Fig.3","The affinity test","Fig.4","Fig.5","Table 2","Fig.6b","Fig.6c","Fig.6a","Fig.6d","Fig.7","Intra,Inter CV ","detection limit"],"not_rendered":{"external_links":2},"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"Table 1","index":0,"n_rows":16,"n_cols":19,"rows_kept":16,"merged":[[1,1,1,3],[1,4,1,6],[1,7,1,9],[1,10,1,12],[1,13,1,15],[1,16,1,18]],"flags":["formulas_cached_only"]}
["Isotype ELISA analysis"]
[null,"NC-1 OD",null,null,"NC-2 OD",null,null,"NC-3 OD",null,null,"NC-4 OD",null,null,"NC-5 OD",null,null,"NC-6 OD"]
["IgG1",0.061,0.063,0.067,1.791,1.871,1.879,1.767,1.773,1.771,0.081,0.087,0.084,0.083,0.085,0.087,1.617,1.671,1.615]
["IgG2a",1.531,1.537,1.542,0.061,0.063,0.065,0.071,0.069,0.067,0.067,0.061,0.063,0.059,0.057,0.056,0.087,0.087,0.088]
["IgG2b",0.051,0.061,0.057,0.083,0.081,0.087,0.057,0.061,0.059,2.041,2.047,2.046,2.727,2.673,2.714,0.071,0.077,0.075]
["IgG3",0.067,0.073,0.071,0.073,0.077,0.075,0.059,0.058,0.057,0.073,0.077,0.074,0.073,0.077,0.079,0.078,0.083,0.081]
["IgA",0.071,0.067,0.069,0.081,0.082,0.088,0.061,0.067,0.063,0.093,0.095,0.097,0.061,0.067,0.064,0.063,0.067,0.066]
["IgM",0.053,0.051,0.054,0.067,0.063,0.061,0.063,0.061,0.057,0.057,0.051,0.054,0.073,0.072,0.071,0.081,0.087,0.086]
[null,"NC-1",null,"NC-2",null,"NC-3",null,"NC-4",null,"NC-5",null,"NC-6"]
[null,"average","SD","average","SD","average","SD","average","SD","average","SD","average","SD"]
["IgG1",0.064,0.003,1.847,0.049,1.77,0.003,0.084,0.003,0.085,0.002,1.634,0.032]
["IgG2a",1.537,0.006,0.063,0.002,0.069,0.002,0.064,0.003,0.057,0.002,0.087,{"v":0.001,"d":"1.00E-03"}]
["IgG2b",0.056,0.005,0.084,0.003,0.059,0.002,2.045,0.003,2.705,0.023,0.074,0.003]
["IgG3",0.07,0.003,0.075,0.002,0.058,{"v":0.001,"d":"1.00E-03"},0.075,0.002,0.076,0.003,0.081,0.003]
["IgA",0.069,0.002,0.084,0.004,0.064,0.003,0.095,0.002,0.064,0.003,0.065,0.002]
["IgM",0.053,0.002,0.064,0.003,0.06,0.003,0.054,0.003,0.072,{"v":0.001,"d":"1.00E-03"},0.0847,0.003]

{"sheet":"Fig.3","index":1,"n_rows":39,"n_cols":25,"rows_kept":39,"flags":["formulas_cached_only"]}
["Titration of canine NGAL-specific mAbs by indirect ELISA"]
["Ascite dilution ","NC-1",null,null,"NC-2",null,null,"NC-3",null,null,"NC-4",null,null,"NC-5",null,null,"NC-6",null,null,"Negative"]
[1000,3.152,3.147,3.156,3.514,3.531,3.492,3.055,3.043,3.067,2.557,2.539,2.568,2.215,2.117,2.312,1.878,1.865,1.891,0.081,0.085,0.082,0.076,0.089,0.079]
[2000,3.106,3.126,3.004,3.509,3.501,3.511,3.062,3.035,3.093,2.543,2.517,2.572,2.199,2.187,2.213,1.863,1.851,1.881,0.087,0.081,0.078,0.079,0.086,0.081]
[4000,3.058,3.059,3.047,3.368,3.351,3.373,2.998,2.987,2.961,2.041,2.101,2.013,1.896,1.905,1.874,1.676,1.693,1.681,0.084,0.086,0.083,0.076,0.08,0.083]
[8000,2.998,3.078,2.967,3.017,3.031,3.009,2.754,2.714,2.804,1.867,1.831,1.907,1.762,1.749,1.776,1.378,1.391,1.359]
[16000,2.625,2.631,2.619,2.778,2.769,2.783,2.553,2.573,2.503,1.764,1.741,1.793,1.335,1.358,1.321,1.184,1.193,1.175]
[32000,2.362,2.357,2.367,2.587,2.589,2.578,2.123,2.313,2.021,1.432,1.402,1.451,1.078,1.081,1.066,0.975,0.962,0.986]
[64000,1.999,1.984,2.002,2.126,2.117,2.132,1.856,1.816,1.872,1.087,1.102,1.061,0.841,0.861,0.831,0.789,0.789,0.789]
[128000,1.541,1.56,1.536,1.878,1.862,1.891,1.412,1.397,1.432,0.814,0.793,0.835,0.627,0.611,0.639,0.541,0.553,0.561]
[256000,1.103,1.097,1.107,1.557,1.527,1.579,1.036,1.041,1.053,0.623,0.617,0.635,0.452,0.469,0.443,0.399,0.387,0.413]
[512000,0.884,0.891,0.887,1.051,1.041,1.062,0.744,0.714,0.754,0.412,0.407,0.421,0.311,0.302,0.32,0.258,0.267,0.245]
[1024000,0.602,0.597,0.611,0.788,0.774,0.801,0.562,0.532,0.592,0.298,0.307,0.281,0.208,0.213,0.201,0.217,0.237,0.201]
[2048000,0.399,0.401,0.394,0.571,0.569,0.575,0.311,0.303,0.327,0.206,0.201,0.21,0.115,0.103,0.122,0.124,0.131,0.14]
[4096000,0.266,0.271,0.264,0.364,0.357,0.367,0.216,0.221,0.204,0.103,0.117,0.951,0.077,0.097,0.062,0.087,0.083,0.077]
[8192000,0.189,0.183,0.192,0.221,0.23,0.217,0.143,0.133,0.151,0.078,0.085,0.072,0.081,0.079,0.067,0.08,0.097,0.081]
[16384000,0.125,0.129,0.119,0.168,0.171,0.164,0.097,0.102,0.091,0.074,0.081,0.071,0.088,0.069,0.072,0.074,0.075,0.069]
[32768000,0.078,0.073,0.083,0.106,0.107,0.113,0.087,0.081,0.092,0.085,0.063,0.097,0.079,0.068,0.07,0.072,0.067,0.082]
[65536000,0.076,0.069,0.081,0.087,0.089,0.085,0.079,0.071,0.089,0.076,0.083,0.069,0.073,0.06,0.059,0.079,0.076,0.073]
["titer","1:4096000",null,null,"1:8192000",null,null,"1:4096000",null,null,"1:2048000",null,null,"1:1024000",null,null,"1:1024000"]
[]
["Ascite dilution (1：1000)","NC-clon1","NC-clon2","NC-clon3","NC-clon4","NC-clon5","NC-clon6"]
[1,38.43902,42.85366,37.2561,31.18293,27.0122,23.02439]
[2,37.87805,42.79268,37.34146,31.0122,26.81707,22.71951]
[4,37.29268,41.07317,36.56098,24.89024,23.12195,20.31707]
[8,36.56098,36.79268,33.58537,22.76829,21.4878,16.80488]
[16,32.0122,33.87805,31.13415,21.5122,16.28049,14.43902]
[32,28.80488,31.54878,25.89024,17.46341,13.14634,11.89024]
[64,24.37805,25.92683,22.63415,13.2561,10.2561,9.62195]
[128,18.79268,22.90244,17.21951,9.92683,7.64634,6.59756]
[256,13.45122,18.9878,12.63415,7.59756,5.5122,4.86585]
[512,10.78049,12.81707,9.07317,5.02439,3.79268,3.14634]
[1024,7.34146,9.60976,6.85366,3.63415,2.53659,2.64634]
[2048,4.86585,6.96341,3.79268,2.5122,1.40244,1.5122]
[4096,3.2439,4.43902,2.63415,1.2561,0.93902,1.06098]
[8192,2.30488,2.69512,1.7439,0.95122,0.9878,1.07317]
[16384,1.52439,2.04878,1.18293,0.90244,1.07317,0.90244]
[32768,0.95122,1.29268,1.06098,1.03659,0.96341,0.87805]
[65536,0.92683,1.06098,0.96341,0.92683,0.89024,0.96341]

{"sheet":"The affinity test","index":2,"n_rows":11,"n_cols":19,"rows_kept":11,"merged":[[1,1,1,3],[1,4,1,6],[1,7,1,9],[1,10,1,12],[1,13,1,15],[1,16,1,18]],"flags":["formulas_cached_only"]}
[]
["Competitive antigen concentration\n（10-9mol）","Nc-1 (OD value)",null,null,"NC-2 (OD value)",null,null,"NC-3 (OD value)",null,null,"NC-4 (OD value)",null,null,"NC-5 (OD value)",null,null,"NC-6 (OD value)"]
[128,0.085,0.076,{"v":0.08180000126361799,"d":"0.082"},0.078,0.079,{"v":0.0819654294,"d":"0.082"},0.096,0.107,{"v":0.116349999696016,"d":"0.116"},0.178,0.201,{"v":0.19204999816417695,"d":"0.192"},0.299,0.317,{"v":0.327299995183944,"d":"0.327"},0.784,0.773,{"v":0.7057000044584274,"d":"0.706"}]
[64,0.166,0.157,{"v":0.163599994421006,"d":"0.164"},0.125,0.127,{"v":0.137799993157386,"d":"0.138"},0.175,0.187,{"v":0.17499529123400004,"d":"0.175"},0.336,0.367,{"v":0.35284998857975,"d":"0.353"},0.545,0.573,{"v":0.5654999303817799,"d":"0.565"},0.955,0.917,{"v":0.95390007019042,"d":"0.954"}]
[32,0.321,0.317,{"v":0.2781000235080719,"d":"0.278"},0.278,0.271,{"v":0.2609999821782112,"d":"0.261"},0.312,0.331,{"v":0.290799998521804,"d":"0.291"},0.568,0.572,{"v":0.578499997377396,"d":"0.578"},0.818,0.819,{"v":0.8786500282287598,"d":"0.879"},1.122,1.201,{"v":1.1094999997615813,"d":"1.109"}]
[16,0.385,0.357,{"v":0.3699000084400177,"d":"0.370"},0.356,0.371,{"v":0.3368500086069107,"d":"0.337"},0.459,0.479,{"v":0.48015001153945996,"d":"0.480"},0.885,0.893,{"v":0.8294994735718,"d":"0.829"},1.126,1.137,{"v":1.0810999741554261,"d":"1.081"},1.199,1.231,{"v":1.218449951171875,"d":"1.218"}]
[8,0.456,0.427,{"v":0.43830001378059386,"d":"0.438"},0.425,0.447,{"v":0.4111500054597855,"d":"0.411"},0.514,0.547,{"v":0.48600009536739996,"d":"0.486"},1.066,1.071,{"v":1.05505001211166,"d":"1.055"},1.247,1.277,{"v":1.3425000100135802,"d":"1.343"},1.269,1.281,{"v":1.2766499457359315,"d":"1.277"}]
[4,0.598,0.537,{"v":0.5617999930381775,"d":"0.562"},0.554,0.567,{"v":0.5522000095844268,"d":"0.552"},0.721,0.717,{"v":0.6839500346183801,"d":"0.684"},1.195,1.197,1.215,1.449,1.487,{"v":1.4537000069618224,"d":"1.454"},1.312,1.327,{"v":1.29330005216598,"d":"1.293"}]
[2,0.812,0.787,{"v":0.791900027275086,"d":"0.792"},0.751,0.791,{"v":0.7406999936103821,"d":"0.741"},0.856,0.867,{"v":0.8156999650001601,"d":"0.816"},1.414,1.473,{"v":1.43484995222092,"d":"1.435"},1.558,1.572,{"v":1.5629999918937683,"d":"1.563"},{"v":1.33,"d":"1.330"},1.317,{"v":1.32864996910096,"d":"1.329"}]
[1,0.987,0.971,{"v":0.955999970912934,"d":"0.956"},0.952,0.979,{"v":0.93489999580384,"d":"0.935"},1.156,1.177,{"v":1.0528999867439202,"d":"1.053"},1.663,1.679,{"v":1.67706499838828,"d":"1.677"},1.753,1.791,{"v":1.7746000499725343,"d":"1.775"},1.412,1.431,{"v":1.3781999950408936,"d":"1.378"}]
[0,1.203,1.171,{"v":1.1900000066757201,"d":"1.190"},1.526,1.534,{"v":1.5501999487876892,"d":"1.550"},1.332,1.371,{"v":1.2874999814033508,"d":"1.287"},1.678,1.679,{"v":1.6769000024795533,"d":"1.677"},1.956,1.937,{"v":1.9034500422477723,"d":"1.903"},1.523,1.541,1.485]

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["checkerboard ELISA for the best pair antibodies"]
[null,null,"capture antibody"]
["detection antibody",null,"NC-1","NC-2","NC-3","NC-4","NC-5","NC-6"]
["HRP-NC-1","positive serum","/",0.582,"/",1.579,0.765,0.352]
[{"$nulls":3},0.574,null,1.595,0.717,0.34199999999999997]
[{"$nulls":3},0.58,null,1.583,0.738,0.35]
[null,"negative serum",null,0.084,null,0.227,0.092,0.093]
[{"$nulls":3},0.082,null,0.267,0.096,0.091]
[{"$nulls":3},0.082,null,0.248,0.094,0.092]
["HRP-NC-2","positive serum",0.882,"/",1.062,"/","/","/"]
[null,null,0.86,null,1.0519999999999998]
[null,null,0.871,null,1.06]
[null,"negative serum",0.071,null,0.071]
[null,null,0.06700000000000002,null,0.06500000000000002]
[null,null,0.069,null,0.067]
["HRP-NC-3","positive serum","/",1.692,"/",3.224,2.721,0.864]
[{"$nulls":3},1.6820000000000002,null,3.026,2.761,0.85]
[{"$nulls":3},1.69,null,3.187,2.731,0.861]
[null,"negative serum",null,0.099,null,0.163,0.081,0.076]
[{"$nulls":3},0.10699999999999998,null,0.151,0.077,0.072]
[{"$nulls":3},0.103,null,0.157,0.078,0.073]
["HRP-NC-4","positive serum",1.22,"/",2.103,"/","/","/"]
[null,null,1.204,null,2.0109999999999997]
[null,null,1.217,null,2.107]
[null,"negative serum",0.312,null,0.097]
[null,null,0.296,null,0.11299999999999999]
[null,null,0.308,null,0.106]
["HRP-NC-5","positive serum",0.794,"/",2.162,"/","/","/"]
[null,null,0.776,null,2.15]
[null,null,0.781,null,2.157]
[null,"negative serum",0.13,null,0.091]
[null,null,0.116,null,0.083]
[null,null,0.125,null,0.087]
["HRP-NC-6","positive serum",0.833,"/",0.96,"/","/","/"]
[null,null,0.849,null,0.95]
[null,null,0.841,null,0.952]
[null,"negative serum",0.097,null,0.081]
[null,null,0.104,null,0.078]
[null,null,0.109,null,0.075]

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["Fig. 5 Checkerboard titration of the antibody pair for simplified sandwich ELISA"]
[null,null," capture antibody (NC-5)"]
["detection antibody (NC-3)",null,"0.5ug/ml","1ug/ml","2ug/ml","4ug/ml"]
["1:1000","positive serum",0.99,1.661,2.518,2.627]
[null,null,0.98,1.6549999999999998,2.548,2.567]
[null,null,0.972,1.671,2.562,2.589]
[null,"negative serum",0.22,0.271,0.254,0.246]
[null,null,0.208,0.235,0.28400000000000003,0.20800000000000002]
[null,null,0.211,0.242,0.278,0.226]
["1:2000","positive serum",0.886,1.559,2.214,2.233]
[null,null,0.86,1.5730000000000002,2.036,2.179]
[null,null,0.871,1.579,2.178,2.217]
[null,"negative serum",0.199,0.177,0.184,0.153]
[null,null,0.187,0.15500000000000003,0.19,0.165]
[null,null,0.193,0.167,0.186,0.157]
["1:4000","positive serum",0.781,1.364,1.917,1.908]
[null,null,0.772,1.392,1.971,1.926]
[null,null,0.769,1.344,1.857,1.9]
[null,"negative serum",0.135,0.13,0.147,0.155]
[null,null,0.127,0.122,0.143,0.149]
[null,null,0.13,0.127,0.145,0.153]
["1:8000","positive serum",0.705,1.188,1.557,1.612]
[null,null,0.732,1.173,1.514,1.6]
[null,null,0.691,1.1199999999999999,1.5550000000000002,1.5739999999999998]
[null,"negative serum",0.089,0.099,0.099,0.098]
[null,null,0.093,0.094,0.093,0.091]
[null,null,0.095,0.093,0.103,0.10999999999999999]
[]
[null,"0.5 ug/ml",null,"1 ug/ml",null,"2 ug/ml",null,"4 ug/ml"]
[null,"average","SD","average","SD","average","SD","average","SD"]
["1:1000",4.583,0.042,6.57,0.032,9.452,0.084,11.429,0.134]
["1:2000",4.52,0.068,9.46,0.062,11.458,0.503,13.897,0.174]
["1:4000",5.908,0.048,10.847,0.191,13.207,0.393,12.575,0.088]
["1:8000",7.71,0.227,12.087,0.372,15.576,0.245,15.34,0.187]

{"sheet":"Table 2","index":5,"n_rows":23,"n_cols":11,"rows_kept":23,"flags":["formulas_cached_only"]}
["The additivity assay"]
[null,"repeat1","repeat2","repeat3","average"]
["NG-clon1",0.875,0.837,0.842,0.8513333333333333]
["NG-clon2",0.996,0.9319999999999999,0.975,0.9676666666666667,1.819]
["NG-clon3",0.98,0.954,0.973,0.969,1.8203333333333331,1.9366666666666665]
["NG-clon4",0.862,0.85,0.843,0.8516666666666666,1.7029999999999998,1.8193333333333332,1.8206666666666664]
["NG-clon5",0.985,0.957,0.975,0.9723333333333333,1.8236666666666665,1.94,1.9413333333333331,1.8239999999999998]
["NG-clon6",0.892,0.882,0.897,0.8903333333333334,1.7416666666666667,1.858,1.8593333333333333,1.742,1.8626666666666667]
["NG-clon1+NG-clon2",1.688,1.6900000000000002,1.684,1.6873333333333334,3.3746666666666667,{"$nulls":4},{"v":0.8552318123511087,"d":"85.52%"}]
["NG-clon1+NG-clon3",0.942,0.9280000000000002,0.937,0.9356666666666668,1.8713333333333335,{"$nulls":4},{"v":0.028016846731368172,"d":"2.80%"}]
["NG-clon1+NG-clon4",1.499,1.439,1.466,1.468,2.936,{"$nulls":4},{"v":0.7240164415736936,"d":"72.40%"}]
["NG-clon1+NG-clon5",1.702,1.6320000000000001,1.678,1.6706666666666667,3.3413333333333335,{"$nulls":4},{"v":0.8322061780296108,"d":"83.22%"}]
["NG-clon1+NG-clon6",1.489,1.5090000000000001,1.501,1.499666666666667,2.999333333333334,{"$nulls":4},{"v":0.722105263157895,"d":"72.21%"}]
["NG-clon2+NG-clon3",1.596,1.576,1.587,1.5863333333333334,3.1726666666666667,{"$nulls":4},{"v":0.6382099827882961,"d":"63.82%"}]
["NG-clon2+NG-clon4",0.991,0.977,0.987,0.985,1.97,{"$nulls":4},{"v":0.08281421766214736,"d":"8.28%"}]
["NG-clon2+NG-clon5",1.012,1.1280000000000001,1.117,1.0856666666666668,2.1713333333333336,{"$nulls":4},{"v":0.1906415646134163,"d":"19.06%"}]
["NG-clon2+NG-clon6",0.867,0.883,0.874,0.8746666666666667,1.7493333333333334,{"$nulls":4},{"v":0.004401913875598185,"d":"0.44%"}]
["NG-clon3+NG-clon4",1.725,1.693,1.717,1.7116666666666667,3.4233333333333333,{"$nulls":4},{"v":0.8802636396924206,"d":"88.03%"}]
["NG-clon3+NG-clon5",1.766,1.7439999999999998,1.757,1.7556666666666665,3.511333333333333,{"$nulls":4},{"v":0.8087225274725274,"d":"80.87%"}]
["NG-clon3+NG-clon6",1.668,1.65,1.637,1.6516666666666666,3.3033333333333332,{"$nulls":4},{"v":0.7766224453209036,"d":"77.66%"}]
["NG-clon4+NG-clon5",1.102,0.9519999999999997,1.017,1.0236666666666665,2.047333333333333,{"$nulls":4},{"v":0.12244152046783618,"d":"12.24%"}]
["NG-clon4+NG-clon6",0.931,0.871,0.916,0.906,1.812,{"$nulls":4},{"v":0.04018369690011481,"d":"4.02%"}]
["NG-clon5+NG-clon6",1.031,1.019,1.026,1.0253333333333332,2.0506666666666664,{"$nulls":4},{"v":0.10093056549749457,"d":"10.09%"}]

{"sheet":"Fig.6b","index":6,"n_rows":27,"n_cols":9,"rows_kept":27,"merged":[[1,2,1,3],[3,0,8,0],[3,1,5,1],[6,1,8,1],[9,0,14,0],[9,1,11,1],[12,1,14,1],[15,0,20,0],[15,1,17,1],[18,1,20,1],[21,0,26,0],[21,1,23,1],[24,1,26,1]],"flags":["formulas_cached_only"]}
["optimize the blocking buffer"]
[null,null,"blocking buffer"]
["times (h)",null,"5% BSA","1% casein",null,"5% BSA",null,"1% casein"]
["1h","positive serum",1.811,1.902,null,"average","SD","average","SD"]
[null,null,1.7970000000000002,1.8760000000000001,null,15.638,0.105,19.502,0.142]
[null,null,1.787,1.897,null,20.018,0.16,21.858,0.093]
[null,"negative serum",0.118,0.098,null,21.231,0.037,24.511,0.089]
[null,null,0.11200000000000002,0.096,null,22.137,0.098,25.009,0.092]
[null,null,0.117,0.093]
["2h","positive serum",1.881,1.897]
[null,null,1.8670000000000002,1.9109999999999998]
[null,null,1.897,1.897]
[null,"negative serum",0.095,0.087]
[null,null,0.093,0.085]
[null,null,0.091,0.083]
["3h","positive serum",1.872,1.943]
[null,null,1.8659999999999999,1.9289999999999998]
[null,null,1.867,1.937]
[null,"negative serum",0.089,0.08]
[null,null,0.087,0.078]
[null,null,0.085,0.079]
["4h","positive serum",1.891,1.932]
[null,null,1.875,1.9180000000000001]
[null,null,1.879,1.927]
[null,"negative serum",0.088,0.08]
[null,null,0.085,0.076]
[null,null,0.08200000000000002,0.074]

{"sheet":"Fig.6c","index":7,"n_rows":8,"n_cols":5,"rows_kept":8,"merged":[[2,0,4,0],[5,0,7,0]],"flags":["formulas_cached_only"]}
["antigen react time"]
["times","30min","45min","60min","90min"]
["positive serum",1.694,1.992,1.998,2.115]
[null,1.679,1.997,2.017,2.11]
[null,1.642,1.976,2.034,2.0889999999999995]
["negative serum",0.067,0.068,0.075,0.079]
[null,0.065,0.061,0.078,0.081]
[null,0.063,0.062,0.083,0.083]

{"sheet":"Fig.6a","index":8,"n_rows":8,"n_cols":4,"rows_kept":8,"merged":[[2,0,4,0],[5,0,7,0]],"flags":["formulas_cached_only"]}
["blocking buffer"]
["enveloped buffer","50mM carbonate（pH9.6）","50mM Tris-HCl（pH8.0）","50mM  PBS（pH7.4）"]
["positive serum",1.579,1.839,1.477]
[null,1.612,1.898,1.415]
[null,1.6059999999999999,1.885,1.403999999999999]
["negative serum",0.091,0.089,0.102]
[null,0.089,0.092,0.099]
[null,0.09600000000000003,0.08900000000000002,0.11700000000000002]

{"sheet":"Fig.6d","index":9,"n_rows":26,"n_cols":4,"rows_kept":26,"merged":[[2,0,7,0],[2,1,4,1],[5,1,7,1],[8,0,13,0],[8,1,10,1],[11,1,13,1],[14,0,19,0],[14,1,16,1],[17,1,19,1],[20,0,25,0],[20,1,22,1],[23,1,25,1]],"flags":["formulas_cached_only"]}
["The optimal conditions HRP-conjugated antibody "]
["Times",null,"PBST+5%BSA","PBST+1%casein"]
["30min","positive serum",1.581,1.618]
[null,null,1.579,1.627]
[null,null,1.533,1.628]
[null,"negative serum",0.069,0.061]
[null,null,0.07100000000000001,0.05500000000000001]
[null,null,0.067,0.057]
["45min","positive serum",2.001,2.076]
[null,null,1.997,2.061]
[null,null,1.9930000000000003,2.0500000000000003]
[null,"negative serum",0.079,0.068]
[null,null,0.071,0.06]
[null,null,0.075,0.063]
["60min","positive serum",2.102,2.115]
[null,null,2.097,2.107]
[null,null,1.9300000000000002,2.0969999999999995]
[null,"negative serum",0.091,0.078]
[null,null,0.087,0.08]
[null,null,0.089,0.077]
["90min","positive serum",2.155,2.123]
[null,null,2.137,2.117]
[null,null,2.1149999999999998,2.093]
[null,"negative serum",0.111,0.102]
[null,null,0.109,0.097]
[null,null,0.107,0.09400000000000001]

{"sheet":"Fig.7","index":10,"n_rows":12,"n_cols":3,"rows_kept":12,"flags":["formulas_cached_only"]}
[]
["The standard curve"]
["recombinant canine NGAL(ng/ml)","log10(rNGAL)","OD450"]
[1600,3.2041199826559246,3.158]
[800,2.9030899869919433,2.789]
[400,2.602059991327962,2.336]
[200,2.301029995663981,1.877]
[100,2,1.362]
[50,1.6989700043360185,0.957]
[25,1.3979400086720375,0.549]
[12.5,1.0969100130080565,0.233]
[6.25,0.7958800173440752,0.113]

{"sheet":"Intra,Inter CV ","index":11,"n_rows":78,"n_cols":13,"rows_kept":78,"flags":["formulas_cached_only"]}
["Intra-assay coefficient","Intra-assay coefficient"]
["n","X1(Low concentration)","log 10X1","OD",null,"X2 (high concentration) ","Log X2","OD"]
[1,13.075817266796992,1.1164688427299703,0.295,null,142.66014812741082,2.1543026706231454,1.694]
[2,14.88842181740076,1.1728486646884273,0.371,null,153.79549710472693,2.1869436201780412,1.738]
[3,14.536602268795582,1.1624629080118694,0.357,null,140.00464130068687,2.146142433234421,1.683]
[4,15.832696841584436,1.1995548961424332,0.407,null,153.00939603558066,2.184718100890208,1.735]
[5,13.12056455136586,1.1179525222551927,0.297,null,160.231454303959,2.204747774480712,1.762]
[6,12.70157470151097,1.1038575667655786,0.278,null,141.93096361239722,2.152077151335311,1.691]
[7,14.837645309634157,1.1713649851632046,0.369,null,147.11460375150165,2.167655786350148,1.712]
[8,14.787041973596066,1.169881305637982,0.367,null,148.63011880060225,2.172106824925816,1.718]
[9,15.832696841584436,1.1995548961424332,0.407,null,142.17361005049315,2.1528189910979227,1.692]
[10,14.736611218689616,1.1683976261127595,0.365,null,147.3661121868962,2.1683976261127595,1.713]
[11,14.812322032108645,1.1706231454005933,0.368,null,147.87041973596064,2.1698813056379818,1.715]
[12,12.942488988470679,1.1120178041543025,0.289,null,151.96750799257734,2.181750741839762,1.731]
[13,14.837645309634157,1.1713649851632046,0.369,null,144.87025630851417,2.160979228486647,1.703]
[14,13.053500885551177,1.115727002967359,0.294,null,150.4179630739865,2.177299703264095,1.725]
[15,13.03122259147511,1.1149851632047476,0.293,null,140.00464130068687,2.146142433234421,1.683]
[16,16.160581110117896,1.2084569732937684,0.419,null,149.64912572947821,2.175074183976261,1.722]
[17,13.03122259147511,1.1149851632047476,0.293,null,148.37645309634172,2.171364985163205,1.717]
[18,16.023145430395903,1.204747774480712,0.414,null,162.7137792747069,2.2114243323442135,1.771]
[19,16.10546594194799,1.206973293768546,0.417,null,163.27060869774937,2.2129080118694358,1.773]
[20,13.03122259147511,1.1149851632047476,0.293,null,166.0834697975289,2.2203264094955486,1.783]
["average",14.368924513180534,{"$nulls":3},150.1070385140893]
["SD",1.2489006784785464,{"$nulls":3},7.810876999382607]
["CV",0.08691678192984706,{"$nulls":3},0.052035381396519025]
[]
["inter-assay coefficients"]
["n"]
[1,20180412,"X1","LogX","OD",null,"X2","LogX2","OD",null,null,"X1","X2"]
[null,null,13.623007541274138,1.1342729970326408,0.319,null,154.05842725483546,2.1876854599406528,1.739,null,null,13.654566061734,154.14712025891808]
[null,null,13.530244292514604,1.1313056379821957,0.315,null,153.53301569517154,2.18620178041543,1.737,null,null,16.00507020377486,156.7136466187252]
[null,null,13.810446351413256,1.140207715133531,0.327,null,154.84991782674714,2.1899109792284865,1.742,null,null,12.529709329707892,135.0748077013426]
[null,"average",13.654566061734,{"$nulls":3},154.14712025891808,{"$nulls":4},15.436083318867922,161.2836488876614]
[{"$nulls":11},12.529879789009234,129.0612689485454]
[2,20180416,15.968498982413013,1.2032640949554894,0.412,null,156.44511894773737,2.1943620178041545,1.748,null,null,13.439419708051886,149.74896131797777]
[null,null,16.10546594194799,1.206973293768546,0.417,null,156.17811545210483,2.193620178041543,1.747,null,null,14.71158914956481,131.73557963757813]
[null,null,15.941245686963578,1.2025222551928783,0.411,null,157.51770545633337,2.197329376854599,1.752,null,null,15.933289754611998,161.15224496184416]
[null,"average",16.00507020377486,{"$nulls":3},156.7136466187252,{"$nulls":4},16.0325418891401,132.18767449671506]
[{"$nulls":11},14.914237825596977,148.66573820551307]
[3,20180508,12.658256369024881,1.102373887240356,0.276,null,133.92282424430385,2.126854599406528,1.657,null,null,14.518638703005971,145.97706910348205]
[null,null,12.380265839646066,1.0927299703264093,0.263,null,135.07152344698264,2.130563798219584,1.662,null,"SD",1.3888600226846741,12.762916405020365]
[null,null,12.550605780452726,1.0986646884272995,0.271,null,136.2300754127413,2.1342729970326406,1.667,null,"CV",0.09566048519391294,0.08743096764035473]
[null,"average",12.529709329707892,{"$nulls":3},135.0748077013426]
[]
[4,20180516,15.09327144857419,1.1787833827893175,0.379,null,165.80001661406786,2.2195845697329375,1.782]
[null,null,15.914038904471525,1.201780415430267,0.41,null,156.44511894773737,2.1943620178041545,1.748]
[null,null,15.30093960355805,1.1847181008902077,0.387,null,161.60581110117897,2.208456973293768,1.767]
[null,"average",15.436083318867922,{"$nulls":3},161.2836488876614]
[]
[5,20180521,12.550605780452726,1.0986646884272995,0.271,null,130.53500885551173,2.115727002967359,1.642]
[null,null,12.359136549846065,1.0919881305637982,0.262,null,128.5435959986339,2.109050445103857,1.633]
[null,null,12.679897036728914,1.1031157270029674,0.277,null,128.10520199149065,2.107566765578635,1.631]
[null,"average",12.529879789009234,{"$nulls":3},129.0612689485454]
[]
[6,20180605,13.210519039032954,1.1209198813056378,0.301,null,152.22731299623683,2.1824925816023737,1.732]
[null,null,13.438112697249677,1.1283382789317506,0.311,null,147.11460375150165,2.167655786350148,1.712]
[null,null,13.669627387873025,1.1357566765578633,0.321,null,149.9049672061948,2.175816023738872,1.723]
[null,"average",13.439419708051886,{"$nulls":3},149.74896131797777]
[]
[7,20180614,14.636265099948204,1.1654302670623145,0.361,null,133.46608454356408,2.1253709198813056,1.655]
[null,null,14.71146037515016,1.1676557863501482,0.364,null,130.53500885551173,2.115727002967359,1.642]
[null,null,14.787041973596066,1.169881305637982,0.367,null,131.20564551365862,2.1179525222551927,1.645]
[null,"average",14.71158914956481,{"$nulls":3},131.73557963757813]
[]
[8,20180623,15.671257891492102,1.1951038575667654,0.401,null,160.77978886204667,2.206231454005935,1.764]
[null,null,16.023145430395903,1.204747774480712,0.414,null,162.99195619935583,2.212166172106825,1.772]
[null,null,16.10546594194799,1.206973293768546,0.417,null,159.68498982413,2.203264094955489,1.76]
[null,"average",15.933289754611998,{"$nulls":3},161.15224496184416]
[]
[null,20180703,15.995798870338824,1.204005934718101,0.413,null,132.10519039032957,2.120919881305638,1.649]
[null,null,15.941245686963578,1.2025222551928783,0.411,null,133.92282424430385,2.126854599406528,1.657]
[null,null,16.160581110117896,1.2084569732937684,0.419,null,130.53500885551173,2.115727002967359,1.642]
[null,"average",16.0325418891401,{"$nulls":3},132.18767449671506]
[]
[null,20180714,15.04179630739864,1.1772997032640948,0.377,null,144.37618072516372,2.159495548961424,1.701]
[null,null,14.787041973596066,1.169881305637982,0.367,null,152.22731299623683,2.1824925816023737,1.732]
[null,null,14.913875195796228,1.1735905044510384,0.372,null,149.3937208951386,2.1743323442136497,1.721]
[null,"average",14.914237825596977,{"$nulls":3},148.66573820551307]

{"sheet":"detection limit","index":12,"n_rows":24,"n_cols":7,"rows_kept":24,"merged":[[0,0,0,2]],"flags":["formulas_cached_only"]}
["The standard curve",null,null,"blank","OD","X","Calculated concentration"]
["recombinant canine NGAL(ng/ml)","log10(rNGAL)","OD450",1,0.013,0.9072700296735904,8.077370978558793]
[1600,{"v":3.2041199826559246,"d":"3.204"},3.158,2,0.011,0.9057863501483678,8.049823351600299]
[800,{"v":2.9030899869919433,"d":"2.903"},2.789,3,0.013,0.9072700296735904,8.077370978558793]
[400,{"v":2.602059991327962,"d":"2.602"},2.336,4,0.005,0.9013353115727001,7.9677428921994835]
[200,{"v":2.301029995663981,"d":"2.301"},1.877,5,0.007,0.9028189910979227,7.995009628289025]
[100,{"v":2,"d":"2.000"},1.362,6,0.012,0.9065281899109792,8.063585401218315]
[50,{"v":1.6989700043360185,"d":"1.699"},0.957,7,0.011,0.9057863501483678,8.049823351600299]
[25,{"v":1.3979400086720375,"d":"1.398"},0.549,8,0.004,0.900593471810089,7.954144416140747]
[12.5,1.0969100130080565,0.233,9,0.012,0.9065281899109792,8.063585401218315]
[6.25,0.7958800173440752,0.113,10,0.028,0.9183976261127595,8.287005481532882]
[{"$nulls":3},11,0.014,0.9080118694362017,8.09118012384494]
[{"$nulls":3},12,0.027,0.9176557863501482,8.272862122847252]
[{"$nulls":3},13,0.025,0.9161721068249257,8.244647779313421]
[{"$nulls":3},14,0.019,0.911721068249258,8.160580782092989]
[{"$nulls":3},15,0.017,0.9102373887240355,8.132749370641601]
[{"$nulls":3},16,0.006,0.9020771513353115,7.981364616333874]
[{"$nulls":3},17,0.015,0.9087537091988129,8.10501287736872]
[{"$nulls":3},18,0.019,0.911721068249258,8.160580782092989]
[{"$nulls":3},19,0.011,0.9057863501483678,8.049823351600299]
[{"$nulls":3},20,0.012,0.9065281899109792,8.063585401218315]
[{"$nulls":4},"average",null,8.092392454413568]
[{"$nulls":4},"SD",null,0.09446574988865249]
[{"$nulls":4},"detection limit",null,8.281323954190873]